Plasmid_Backbone

Part:BBa_K3200999:Design

Designed by: Choi HyeonSeok   Group: iGEM19_KOREA   (2019-10-15)


hCMV promoter and IgK leader sequence and PDGFR transmembrane domain for membrane protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 5240
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 5246
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 5240
    Illegal BamHI site found at 5122
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 5240
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 5240
    Plasmid lacks a suffix.
    Illegal XbaI site found at 5255
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 1626
    Illegal NgoMIV site found at 1909
    Illegal NgoMIV site found at 4108
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 1414
    Illegal BsaI.rc site found at 3131
    Illegal BsaI.rc site found at 5097
    Illegal SapI site found at 1849


Design Notes

This vector is for mammalian expression and


Source

Invitrogen pDisplay vector

References